Detour: Pitfalls of Assembling Double-Stranded DNA
Understand the difficulties of assembling genomes from double-stranded DNA reads. Learn how reverse complements and inverted repeats complicate de Bruijn graph assembly and why careful handling of these factors is essential in bioinformatics.
We'll cover the following...
We'll cover the following...
Handling reads in double-stranded DNA
To assemble real genomes, bioinformaticians must handle reads from both DNA strands without knowing in advance which strand each read comes from. To address this challenge, they first add the reverse complement of each read to the ...